Novel imaging technologies, optical clearing for large organs and data analysis tools

Novel imaging technologies, optical clearing for large organs and data analysis tools
Theme: Methods and technology development

Wednesday 26th April, 09:30 – 11:10

Histological evaluation of tissue has been crucial to understand function, disease and circuitry. However, traditional histology, imaging and analysis methods are extremely time consuming and possess a major limitation to understand the three-dimensional structure of an entire organ. Deep imaging technologies are necessary to overcome this problem, but imaging a large block of tissue or whole-organ present technical challenges such as tissue opacity, light penetration and scattering, acquisition speed, large data sets obtained, etc. Imaging an entire organ or organism rapidly and at high resolution is the goal that the novel imaging technologies highlighted in this symposium are aiming. This includes whole-organism light-sheet microscopy, serial-sectioning two-photon microscopy and mesoscale imaging. Additionally, newly developed tools to register, map and quantify the large data sets are crucial to go beyond the beautifully technical images to understand the relationship between structure and function.

  • Cristina Martinez-Gonzalez, University of Edinburgh, UK: Whole-brain light sheet imaging of behaviourally-activated neurons in the rat
  • Steven J. West, The Sainsbury Wellcome Centre for Neural Circuits and Behaviour, UK: Automated annotation of histology data with registration to tissue atlases
  • Alain Chedotal, Institut de la Vision, Inserm, France: 3D imaging of the developing human nervous system with tissue clearing and light sheet microscopy 
  • Gail McConnell, University of Strathclyde, UK: Optical mesoscopy with the Mesolens

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